SubtiBank SubtiBank
Version comparison:

2019-06-25 09:23:522025-05-23 18:58:12

locus

BSU01130

BSU_01130

outlinks

bsu

BSU01130

BSU_01130

The protein

Protein family

[SW|TRAFAC class translation factor GTPase superfamily] (according to UniProt)

[SW|TRAFAC class translation factor GTPase superfamily] (according to UniProt)

[SW|Classic translation factor GTPase family] (according to UniProt)

The protein

Modification

phosphorylated on Thr-384 by [[protein|PrkC]], dephosphorylated by [[protein|PrpC]] [Pubmed|19246764], [Pubmed|17726680]

Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [Pubmed|22938038]

phosphorylated on several Arg residues [Pubmed|24263382]

phosphorylated during [SW|sporulation] on Thr-63 by [[protein|YabT]], dephosphorylated by [[protein|PrpC]] [Pubmed|26056311]

phosphorylated on Tyr-270 [Pubmed|26381121]

phosphorylated on Thr-384 by [[protein|PrkC]], dephosphorylated by [[protein|PrpC]] [Pubmed|19246764], [Pubmed|17726680]

Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species [Pubmed|22938038]

phosphorylated on Arg-265 [Pubmed|31221751]

phosphorylated on several Arg residues [Pubmed|24263382]

phosphorylated during [SW|sporulation] on Thr-63 by [[protein|YabT]], dephosphorylated by [[protein|PrpC]] [Pubmed|26056311]

phosphorylated on Tyr-270 [Pubmed|26381121]

Biological materials

Expression vectors

for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP839, available in [SW|Stülke] lab

for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP847, available in [SW|Stülke] lab

References

22938038, 19246764, 20133608, 25707802, 19246764, 17726680, 18763711, 24263382, 15378759, 25676310, 26056311, 26381121, 11918677, 28516784, 30394869

22938038, 19246764, 20133608, 25707802, 19246764, 17726680, 18763711, 24263382, 15378759, 25676310, 26056311, 26381121, 11918677, 28516784, 30394869, 31221751, 32024753, 32949815

The protein

[SW|Domains]

[SW|tr-type G domain] (aa 10-205) (according to UniProt)

Biological materials

Expression vector

for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP839, available in [SW|Stülke] lab

for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP847, available in [SW|Stülke] lab

Biological materials

two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab